Difference between revisions of "YmcB"
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || tRNA methylthiotransferase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873 | ||
| Line 52: | Line 52: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA {{PubMed|20472640}} |
* '''Protein family:''' MiaB subfamily (according to Swiss-Prot) | * '''Protein family:''' MiaB subfamily (according to Swiss-Prot) | ||
| Line 117: | Line 117: | ||
=References= | =References= | ||
| − | + | <pubmed> 20472640 </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:26, 18 May 2010
- Description: tRNA methylthiotransferase
| Gene name | ymcB |
| Synonyms | |
| Essential | no |
| Product | tRNA methylthiotransferase |
| Function | tRNA maturation |
| MW, pI | 57 kDa, 6.873 |
| Gene length, protein length | 1527 bp, 509 aa |
| Immediate neighbours | kbl, ymcA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17010
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA PubMed
- Protein family: MiaB subfamily (according to Swiss-Prot)
- Paralogous protein(s): YqeV
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31778
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1109 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
