Difference between revisions of "RpoE"

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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
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* '''Interactions:''' closely associated to RNA polymerase ([[RpoA]](2)-[[RpoB]]-[[RpoC]]) {{PubMed|20724389}}
  
* '''Localization:'''
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* '''Localization:'''closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons {{PubMed|20724389}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Additional information:'''
 
* '''Additional information:'''
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** present at equimolar levels with RNA polymerase {{PubMed|20724389}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 
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<pubmed>7545758,7599136,7515111,10336502,2843435,17189250, 20310067 ,20724389  </pubmed>
<pubmed>7545758,7599136,7515111,10336502,2843435,17189250, 20310067 , </pubmed>
 
 
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:02, 21 August 2010

  • Description: RNA polymerase delta subunit

Gene name rpoE
Synonyms
Essential no
Product RNA polymerase delta subunit
Function transcription
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 20 kDa, 3.654
Gene length, protein length 519 bp, 173 aa
Immediate neighbours pyrG, acdA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RpoE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU37160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: rpoE family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons PubMed

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • fadF: repressed in the absence of long-chain fatty acids (FadR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • present at equimolar levels with RNA polymerase PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

Your additional remarks

References

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)

Veronika Motácková, Hana Sanderová, Lukás Zídek, Jirí Novácek, Petr Padrta, Alzbeta Svenková, Jana Korelusová, Jirí Jonák, Libor Krásný, Vladimír Sklenár
Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs.
Proteins: 2010, 78(7);1807-10
[PubMed:20310067] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

F J López de Saro, N Yoshikawa, J D Helmann
Expression, abundance, and RNA polymerase binding properties of the delta factor of Bacillus subtilis.
J Biol Chem: 1999, 274(22);15953-8
[PubMed:10336502] [WorldCat.org] [DOI] (P p)

F J López de Saro, A Y Woody, J D Helmann
Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase.
J Mol Biol: 1995, 252(2);189-202
[PubMed:7545758] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: effects of temperature, delta protein, and sigma factor mutations.
Biochemistry: 1995, 34(26);8465-73
[PubMed:7599136] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
The delta subunit of Bacillus subtilis RNA polymerase. An allosteric effector of the initiation and core-recycling phases of transcription.
J Mol Biol: 1994, 239(1);1-14
[PubMed:7515111] [WorldCat.org] [DOI] (P p)

M Lampe, C Binnie, R Schmidt, R Losick
Cloned gene encoding the delta subunit of Bacillus subtilis RNA polymerase.
Gene: 1988, 67(1);13-9
[PubMed:2843435] [WorldCat.org] [DOI] (P p)