Difference between revisions of "CccA"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[respiration]]}}
 
=The protein=
 
=The protein=
  

Revision as of 15:35, 30 November 2010

  • Description: cytochrome c550

Gene name cccA
Synonyms
Essential no
Product cytochrome c550
Function respiration
MW, pI 12 kDa, 5.276
Gene length, protein length 360 bp, 120 aa
Immediate neighbours trmK, sigA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CccA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25190

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

Categories containing this gene/protein

respiration

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
    • CcpA: transcription repression, cre site overlaps -35 region of the promoter PubMed
    • AbrB: transcription repression PubMed
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

V Monedero, G Boël, J Deutscher
Catabolite regulation of the cytochrome c550-encoding Bacillus subtilis cccA gene.
J Mol Microbiol Biotechnol: 2001, 3(3);433-8
[PubMed:11361075] [WorldCat.org] (P p)

P S David, M R Morrison, S L Wong, B C Hill
Expression, purification, and characterization of recombinant forms of membrane-bound cytochrome c-550nm from Bacillus subtilis.
Protein Expr Purif: 1999, 15(1);69-76
[PubMed:10024472] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Physico-chemical characterisation of membrane-bound and water-soluble forms of Bacillus subtilis cytochrome c-550.
Eur J Biochem: 1993, 212(2);499-509
[PubMed:8383048] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis holo-cytochrome c-550 can be synthesised in aerobic Escherichia coli.
FEBS Lett: 1990, 270(1-2);147-51
[PubMed:2171986] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis 13-kilodalton cytochrome c-550 encoded by cccA consists of a membrane-anchor and a heme domain.
J Biol Chem: 1990, 265(23);13939-48
[PubMed:2166045] [WorldCat.org] (P p)