Difference between revisions of "DprA"

From SubtiWiki
Jump to: navigation, search
Line 48: Line 48:
  
  
 +
 +
= Categories containing this gene/protein =
 +
{{SubtiWiki category|[[DNA repair/ recombination]]}},
 +
{{SubtiWiki category|[[genetic competence]]}}
 
=The protein=
 
=The protein=
  

Revision as of 16:24, 30 November 2010

  • Description: conveys incoming ssDNA to RecA

Gene name dprA
Synonyms smf
Essential no
Product recombination mediator protein
Function genetic transformation
MW, pI 32 kDa, 9.755
Gene length, protein length 891 bp, 297 aa
Immediate neighbours sucD, topA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Smf context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: smf family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: dprA (according to DBTBS)
  • Regulation:
    • expressed under conditions that trigger genetic competence (ComK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References

Serkalem Tadesse, Peter L Graumann
DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells.
BMC Microbiol: 2007, 7;105
[PubMed:18045469] [WorldCat.org] [DOI] (I e)

Isabelle Mortier-Barrière, Marion Velten, Pauline Dupaigne, Nicolas Mirouze, Olivier Piétrement, Stephen McGovern, Gwennaele Fichant, Bernard Martin, Philippe Noirot, Eric Le Cam, Patrice Polard, Jean-Pierre Claverys
A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA.
Cell: 2007, 130(5);824-36
[PubMed:17803906] [WorldCat.org] [DOI] (P p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Janet C Lindow, Masayoshi Kuwano, Shigeki Moriya, Alan D Grossman
Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein.
Mol Microbiol: 2002, 46(4);997-1009
[PubMed:12421306] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)