Difference between revisions of "FliE"

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(Expression and regulation)
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|style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU16200 fliE]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 4.486   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 4.486   

Revision as of 09:43, 7 August 2012

  • Description: flagellar hook-basal body protein

Gene name fliE
Synonyms
Essential no
Product flagellar hook-basal body protein
Function movement and chemotaxis
Gene expression levels in SubtiExpress: fliE
MW, pI 11 kDa, 4.486
Gene length, protein length 318 bp, 106 aa
Immediate neighbours flgC, fliF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FliE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FliE expression.png
























Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16200

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliE family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: bacterial flagellum basal body (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996] [WorldCat.org] [DOI] (P p)

L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612] [WorldCat.org] [DOI] (P p)

A R Zuberi, C Ying, D S Bischoff, G W Ordal
Gene-protein relationships in the flagellar hook-basal body complex of Bacillus subtilis: sequences of the flgB, flgC, flgG, fliE and fliF genes.
Gene: 1991, 101(1);23-31
[PubMed:1905667] [WorldCat.org] [DOI] (P p)