Difference between revisions of "Mfd"

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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed>17239578 15063847 17917874 8811173 7968917 15720542 16464004 19023283 22201950</pubmed>
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<pubmed>17239578 15063847 17917874 8811173 7968917 15720542 16464004 19023283 22201950 22749141</pubmed>
  
 
==Original Articles==
 
==Original Articles==

Revision as of 19:06, 25 August 2012

  • Description: transcription-repair coupling factor

Gene name mfd
Synonyms
Essential no
Product transcription-repair coupling factor
Function promotes strand-specific DNA repair by displacing

RNA polymerase stalled at a nucleotide lesion and directing

the (A)BC excinuclease to the RNA damage site

Gene expression levels in SubtiExpress: mfd

RNA polymerase stalled at a nucleotide lesion and directing

the (A)BC excinuclease to the RNA damage site

Interactions involving this protein in SubtInteract: Mfd
MW, pI 133 kDa, 5.367
Gene length, protein length 3531 bp, 1177 aa
Immediate neighbours fin, spoVT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Mfd context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Mfd expression.png
























Categories containing this gene/protein

DNA repair/ recombination, transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00550

Phenotypes of a mutant

In an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
    • is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
  • Protein family:
  • Paralogous protein(s): RecG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 2EYQ (Mfd from E. coli) PubMed
    • 3MLQ (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1167 (del ermC), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • two-hybrid system:
  • Antibody:
  • Strep-tag construct: GP890 (spc, based on pGP1389), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ann Ganesan, Graciela Spivak, Philip C Hanawalt
Transcription-coupled DNA repair in prokaryotes.
Prog Mol Biol Transl Sci: 2012, 110;25-40
[PubMed:22749141] [WorldCat.org] [DOI] (I p)

Eduardo A Robleto, Holly A Martin, Mario Pedraza-Reyes
Mfd and transcriptional derepression cause genetic diversity in Bacillus subtilis.
Front Biosci (Elite Ed): 2012, 4(4);1246-54
[PubMed:22201950] [WorldCat.org] [DOI] (I e)

Philip C Hanawalt, Graciela Spivak
Transcription-coupled DNA repair: two decades of progress and surprises.
Nat Rev Mol Cell Biol: 2008, 9(12);958-70
[PubMed:19023283] [WorldCat.org] [DOI] (I p)

Rodrigo S Galhardo, P J Hastings, Susan M Rosenberg
Mutation as a stress response and the regulation of evolvability.
Crit Rev Biochem Mol Biol: 2007, 42(5);399-435
[PubMed:17917874] [WorldCat.org] [DOI] (P p)

Alexandra M Deaconescu, Nigel Savery, Seth A Darst
The bacterial transcription repair coupling factor.
Curr Opin Struct Biol: 2007, 17(1);96-102
[PubMed:17239578] [WorldCat.org] [DOI] (P p)

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Sergei Borukhov, Jookyung Lee, Oleg Laptenko
Bacterial transcription elongation factors: new insights into molecular mechanism of action.
Mol Microbiol: 2005, 55(5);1315-24
[PubMed:15720542] [WorldCat.org] [DOI] (P p)

Jeffrey Roberts, Joo-Seop Park
Mfd, the bacterial transcription repair coupling factor: translocation, repair and termination.
Curr Opin Microbiol: 2004, 7(2);120-5
[PubMed:15063847] [WorldCat.org] [DOI] (P p)

E C Friedberg
Relationships between DNA repair and transcription.
Annu Rev Biochem: 1996, 65;15-42
[PubMed:8811173] [WorldCat.org] [DOI] (P p)

C P Selby, A Sancar
Mechanisms of transcription-repair coupling and mutation frequency decline.
Microbiol Rev: 1994, 58(3);317-29
[PubMed:7968917] [WorldCat.org] [DOI] (P p)


Original Articles

Additional publications: PubMed