Difference between revisions of "ProA"

From SubtiWiki
Jump to: navigation, search
(Categories containing this gene/protein)
Line 46: Line 46:
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}
+
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
 +
{{SubtiWiki category|[[coping with hyper-osmotic stress]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =

Revision as of 15:11, 2 September 2012

  • Description: glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis

Gene name proA
Synonyms
Essential no
Product glutamate-5-semialdehyde dehydrogenase
Function biosynthesis of proline
Gene expression levels in SubtiExpress: proA
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 45 kDa, 5.098
Gene length, protein length 1245 bp, 415 aa
Immediate neighbours proB, ohrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ProA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ProA expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU13130

Phenotypes of a mutant

  • auxotrophic for proline PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: gamma-glutamyl phosphate reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1O20 (from Thermotoga maritima, 46% identity, 67% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Vivianne J Goosens, Ruben A T Mars, Michiel Akeroyd, Andre Vente, Annette Dreisbach, Emma L Denham, Thijs R H M Kouwen, Tjeerd van Rij, Maurien Olsthoorn, Jan Maarten van Dijl
Is proteomics a reliable tool to probe the oxidative folding of bacterial membrane proteins?
Antioxid Redox Signal: 2013, 18(10);1159-64
[PubMed:22540663] [WorldCat.org] [DOI] (I p)

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)