Difference between revisions of "GlnH"

From SubtiWiki
Jump to: navigation, search
(Categories containing this gene/protein)
(Extended information on the protein)
Line 88: Line 88:
  
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
 +
** K(D) for Gln: 120 myM {{PubMed|23038252}}
  
 
* '''Domains:'''  
 
* '''Domains:'''  

Revision as of 18:32, 8 October 2012

Gene name glnH
Synonyms
Essential no
Product glutamine ABC transporter (binding protein)
Function glutamine uptake
Gene expression levels in SubtiExpress: glnH
Interactions involving this protein in SubtInteract: GlnH
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 29 kDa, 8.549
Gene length, protein length 819 bp, 273 aa
Immediate neighbours glnQ, glnM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlnH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlnH expression.png




























Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, glutamate metabolism, utilization of amino acids, membrane proteins, sporulation proteins

This gene is a member of the following regulons

SigE regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU27440

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed during sporulation (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP734 (spc), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)