Difference between revisions of "CdaA"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
** expression of native ''ybbP'' in ''B. subtilis'': pGP1960 (in [[pBQ200]]), available in [[Stülke]] lab
 
** expression of native ''ybbP'' in ''B. subtilis'': pGP1960 (in [[pBQ200]]), available in [[Stülke]] lab
** expression of ''ybbP''-Strep in ''B. subtilis'' suitable for [[SPINE]]: pGP1986 (in pGP382), available in [[Stülke]] lab
+
** expression of ''ybbP''-Strep in ''B. subtilis'' suitable for [[SPINE]]: pGP1986 (in [[pGP382]]), available in [[Stülke]] lab
 
          
 
          
 
* '''lacZ fusion:''' GP1339 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
 
* '''lacZ fusion:''' GP1339 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab

Revision as of 09:02, 31 October 2012

  • Description: maybe involved in the synthesis of c-di-AMP in vegetative cells

Gene name ybbP
Synonyms ybbQ
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ybbP
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 30 kDa, 8.074
Gene length, protein length 819 bp, 273 aa
Immediate neighbours ybbM, ybbR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbP expression.png


























Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01750

Phenotypes of a mutant

  • inactivation of ybbP results in severe beta-lactam sensitivity PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YojJ

Extended information on the protein

  • Kinetic information:
  • Domains: contains a DAC domain potentially involved in the synthesis of c-di-AMP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the ybbP-ybbR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
    • expression of native ybbP in B. subtilis: pGP1960 (in pBQ200), available in Stülke lab
    • expression of ybbP-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Stülke lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Additional publications: PubMed

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)