Difference between revisions of "YaaH"

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|style="background:#ABCDEF;" align="center"|'''Function''' || survival of ethanol  stress, protection of the spore
 
|style="background:#ABCDEF;" align="center"|'''Function''' || survival of ethanol  stress, protection of the spore
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00160 yaaH]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00160 yaaH]
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.618   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.618   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dgk]]'', ''[[yaaI]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dgk]]'', ''[[yaaI]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11792&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00160 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00160 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00160 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yaaH_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yaaH_context.gif]]

Revision as of 11:53, 13 May 2013

  • Description: general stress protein, survival of ethanol stress, SafA-dependent spore coat protein, spore cortex lytic protein

Gene name yaaH
Synonyms sleL
Essential no
Product N-acetylglucosaminidase
Function survival of ethanol stress, protection of the spore
Gene expression levels in SubtiExpress: yaaH
MW, pI 48 kDa, 5.618
Gene length, protein length 1281 bp, 427 aa
Immediate neighbours dgk, yaaI
Sequences Protein DNA Advanced_DNA
Genetic context
YaaH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YaaH expression.png















Categories containing this gene/protein

sporulation proteins, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU00160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

required for survival of ethanol stress PubMed

Expression and regulation

  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Emily A Lambert, Nora Sherry, David L Popham
In vitro and in vivo analyses of the Bacillus anthracis spore cortex lytic protein SleL.
Microbiology (Reading): 2012, 158(Pt 5);1359-1368
[PubMed:22343356] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Emily A Lambert, David L Popham
The Bacillus anthracis SleL (YaaH) protein is an N-acetylglucosaminidase involved in spore cortex depolymerization.
J Bacteriol: 2008, 190(23);7601-7
[PubMed:18835992] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A Aronson
Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.
Cell Mol Life Sci: 2002, 59(3);417-25
[PubMed:11964120] [WorldCat.org] [DOI] (P p)

T Kodama, H Takamatsu, K Asai, K Kobayashi, N Ogasawara, K Watabe
The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.
J Bacteriol: 1999, 181(15);4584-91
[PubMed:10419957] [WorldCat.org] [DOI] (P p)