Difference between revisions of "ConE"

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|style="background:#ABCDEF;" align="center"|'''Function''' || conjugative transfer of ICEBs1
 
|style="background:#ABCDEF;" align="center"|'''Function''' || conjugative transfer of ICEBs1
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU04940 conE]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04940 conE]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 96 kDa, 5.511   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 96 kDa, 5.511   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yddD]]'', ''[[yddF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yddD]]'', ''[[yddF]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12301&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04940 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04940 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04940 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yddE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yddE_context.gif]]

Revision as of 12:12, 13 May 2013


Gene name conE
Synonyms yddE
Essential no
Product conjugation protein
Function conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: conE
MW, pI 96 kDa, 5.511
Gene length, protein length 2493 bp, 831 aa
Immediate neighbours yddD, yddF
Sequences Protein DNA Advanced_DNA
Genetic context
YddE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ConE expression.png
























Categories containing this gene/protein

mobile genetic elements

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU04940

Phenotypes of a mutant

Defective for conjugal transfer of ICEBs1

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: required for conjugative transfer of ICEBs1, putative ATPase PubMed
  • Protein family: HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • Located at the cell poles, in close association with the membrane

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Melanie Barker Berkmen, Suffolk University, Boston, MA Homepage

Your additional remarks

References

Melanie B Berkmen, Catherine A Lee, Emma-Kate Loveday, Alan D Grossman
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2010, 192(1);38-45
[PubMed:19734305] [WorldCat.org] [DOI] (I p)