Difference between revisions of "HemA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || porphyrin biosynthesis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || porphyrin biosynthesis  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU28170 hemA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28170 hemA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.313   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.313   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemX]]'', ''[[ysxD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemX]]'', ''[[ysxD]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14777&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28170 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:hemA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:hemA_context.gif]]

Revision as of 13:30, 13 May 2013

  • Description: glutamyl-tRNA reductase

Gene name hemA
Synonyms
Essential no
Product glutamyl-tRNA reductase
Function porphyrin biosynthesis
Gene expression levels in SubtiExpress: hemA
MW, pI 50 kDa, 5.313
Gene length, protein length 1365 bp, 455 aa
Immediate neighbours hemX, ysxD
Sequences Protein DNA Advanced_DNA
Genetic context
HemA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HemA expression.png
























Categories containing this gene/protein

biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

  • Locus tag: BSU28170

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH (according to Swiss-Prot)
  • Protein family: glutamyl-tRNA reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

Per Johansson, Lars Hederstedt
Organization of genes for tetrapyrrole biosynthesis in gram--positive bacteria.
Microbiology (Reading): 1999, 145 ( Pt 3);529-538
[PubMed:10217486] [WorldCat.org] [DOI] (P p)

N Bsat, L Chen, J D Helmann
Mutation of the Bacillus subtilis alkyl hydroperoxide reductase (ahpCF) operon reveals compensatory interactions among hydrogen peroxide stress genes.
J Bacteriol: 1996, 178(22);6579-86
[PubMed:8932315] [WorldCat.org] [DOI] (P p)

L Chen, L Keramati, J D Helmann
Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions.
Proc Natl Acad Sci U S A: 1995, 92(18);8190-4
[PubMed:7667267] [WorldCat.org] [DOI] (P p)

I Schröder, P Johansson, L Rutberg, L Hederstedt
The hemX gene of the Bacillus subtilis hemAXCDBL operon encodes a membrane protein, negatively affecting the steady-state cellular concentration of HemA (glutamyl-tRNA reductase).
Microbiology (Reading): 1994, 140 ( Pt 4);731-40
[PubMed:8012594] [WorldCat.org] [DOI] (P p)

I Schröder, L Hederstedt, C G Kannangara, P Gough
Glutamyl-tRNA reductase activity in Bacillus subtilis is dependent on the hemA gene product.
Biochem J: 1992, 281 ( Pt 3)(Pt 3);843-50
[PubMed:1536660] [WorldCat.org] [DOI] (P p)

M Hansson, L Rutberg, I Schröder, L Hederstedt
The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III.
J Bacteriol: 1991, 173(8);2590-9
[PubMed:1672867] [WorldCat.org] [DOI] (P p)