Difference between revisions of "MrgA"

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<br/>protection against oxidative stress  
 
<br/>protection against oxidative stress  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU32990 mrgA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32990 mrgA]
 
<br/>protection against oxidative stress  
 
<br/>protection against oxidative stress  
 
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yusZ]]'', ''[[htrB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yusZ]]'', ''[[htrB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15288&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32990 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mrgA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mrgA_context.gif]]

Revision as of 13:48, 13 May 2013

  • Description: iron storage protein, DNA-binding stress protein, forms highly stable, multimeric protein-DNA complexes which protect against oxidative killing

Gene name mrgA
Synonyms
Essential no
Product mini-ferritin, DNA-binding stress protein
Function iron storage,


protection against oxidative stress

Gene expression levels in SubtiExpress: mrgA


protection against oxidative stress

Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 17 kDa, 4.597
Gene length, protein length 459 bp, 153 aa
Immediate neighbours yusZ, htrB
Sequences Protein DNA Advanced_DNA
Genetic context
MrgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MrgA expression.png




























Categories containing this gene/protein

acquisition of iron, iron metabolism, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

  • Locus tag: BSU32990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: forms highly stable, multimeric protein-DNA complexes which protect against oxidative killing
  • Protein family: dps family (according to Swiss-Prot)
  • Paralogous protein(s): Dps

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Emilia Chiancone, Pierpaolo Ceci, Andrea Ilari, Frederica Ribacchi, Simonetta Stefanini
Iron and proteins for iron storage and detoxification.
Biometals: 2004, 17(3);197-202
[PubMed:15222465] [WorldCat.org] [DOI] (P p)

Original publications

Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

L Casillas-Martinez, P Setlow
Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents.
J Bacteriol: 1997, 179(23);7420-5
[PubMed:9393707] [WorldCat.org] [DOI] (P p)

H Antelmann, S Engelmann, R Schmid, A Sorokin, A Lapidus, M Hecker
Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor sigmaB in Bacillus subtilis.
J Bacteriol: 1997, 179(23);7251-6
[PubMed:9393687] [WorldCat.org] [DOI] (P p)

N Bsat, L Chen, J D Helmann
Mutation of the Bacillus subtilis alkyl hydroperoxide reductase (ahpCF) operon reveals compensatory interactions among hydrogen peroxide stress genes.
J Bacteriol: 1996, 178(22);6579-86
[PubMed:8932315] [WorldCat.org] [DOI] (P p)

L Chen, J D Helmann
Bacillus subtilis MrgA is a Dps(PexB) homologue: evidence for metalloregulation of an oxidative-stress gene.
Mol Microbiol: 1995, 18(2);295-300
[PubMed:8709848] [WorldCat.org] [DOI] (P p)

L Chen, L Keramati, J D Helmann
Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions.
Proc Natl Acad Sci U S A: 1995, 92(18);8190-4
[PubMed:7667267] [WorldCat.org] [DOI] (P p)

L Chen, L P James, J D Helmann
Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.
J Bacteriol: 1993, 175(17);5428-37
[PubMed:8396117] [WorldCat.org] [DOI] (P p)