Difference between revisions of "SacP"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, <br/>control of [[SacT]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, <br/>control of [[SacT]] activity
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38050 sacP]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38050 sacP]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacP SacP]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacP SacP]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacA]]'', ''[[ywcJ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacA]]'', ''[[ywcJ]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15831&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38050 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sacP_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sacP_context.gif]]

Revision as of 14:09, 13 May 2013

Gene name sacP
Synonyms ipa-49d
Essential no
Product trigger enzyme: sucrose-specific
phosphotransferase system, EIIBC component
Function sucrose uptake and phosphorylation,
control of SacT activity
Gene expression levels in SubtiExpress: sacP
Interactions involving this protein in SubtInteract: SacP
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 49 kDa, 7.026
Gene length, protein length 1383 bp, 461 aa
Immediate neighbours sacA, ywcJ
Sequences Protein DNA Advanced_DNA
Genetic context
SacP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SacP expression.png






























Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, SacT regulon

The gene

Basic information

  • Locus tag: BSU38050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag, in pWH844: pGP429, available in Stülke lab
    • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP436, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561] [WorldCat.org] [DOI] (P p)

S L Sutrina, P Reddy, M H Saier, J Reizer
The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease.
J Biol Chem: 1990, 265(30);18581-9
[PubMed:2120236] [WorldCat.org] (P p)

M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394] [WorldCat.org] [DOI] (P p)

A Fouet, M Arnaud, A Klier, G Rapoport
Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria.
Proc Natl Acad Sci U S A: 1987, 84(24);8773-7
[PubMed:3122206] [WorldCat.org] [DOI] (P p)