Difference between revisions of "MecA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbE]]'', ''[[coiA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbE]]'', ''[[coiA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mecA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mecA_context.gif]]

Revision as of 09:55, 14 May 2013

  • Description: buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A∼P by direct interaction

Gene name mecA
Synonyms
Essential no
Product adaptor protein
Function control of ComK degradation, regulation of competence
Gene expression levels in SubtiExpress: mecA
Interactions involving this protein in SubtInteract: MecA
MW, pI 25 kDa, 4.209
Gene length, protein length 654 bp, 218 aa
Immediate neighbours yjbE, coiA
Sequences Protein DNA DNA_with_flanks
Genetic context
MecA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MecA expression.png















Categories containing this gene/protein

genetic competence, proteolysis

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: mecA family (according to Swiss-Prot)
  • Paralogous protein(s): YpbH

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal domain (1 ... 120): recruitment of protein substrates PubMed
    • C-terminal domain (121 ... 218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional reviews: PubMed

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Original Publications

Additonal publications: PubMed

Feng Wang, Ziqing Mei, Yutao Qi, Chuangye Yan, Qi Hu, Jiawei Wang, Yigong Shi
Structure and mechanism of the hexameric MecA-ClpC molecular machine.
Nature: 2011, 471(7338);331-5
[PubMed:21368759] [WorldCat.org] [DOI] (I p)

Feng Wang, Ziqing Mei, Yutao Qi, Chuangye Yan, Siheng Xiang, Zhiyuan Zhou, Qi Hu, Jiawei Wang, Yigong Shi
Crystal structure of the MecA degradation tag.
J Biol Chem: 2009, 284(49);34376-81
[PubMed:19801546] [WorldCat.org] [DOI] (I p)

Ziqing Mei, Feng Wang, Yutao Qi, Zhiyuan Zhou, Qi Hu, Han Li, Jiawei Wu, Yigong Shi
Molecular determinants of MecA as a degradation tag for the ClpCP protease.
J Biol Chem: 2009, 284(49);34366-75
[PubMed:19767395] [WorldCat.org] [DOI] (I p)

Douglas J Kojetin, Patrick D McLaughlin, Richele J Thompson, David Dubnau, Peter Prepiak, Mark Rance, John Cavanagh
Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions.
J Mol Biol: 2009, 387(3);639-52
[PubMed:19361434] [WorldCat.org] [DOI] (I p)

Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088] [WorldCat.org] [DOI] (P p)

Peter Prepiak, David Dubnau
A peptide signal for adapter protein-mediated degradation by the AAA+ protease ClpCP.
Mol Cell: 2007, 26(5);639-47
[PubMed:17560370] [WorldCat.org] [DOI] (P p)

Michiko M Nakano, Shunji Nakano, Peter Zuber
Spx (YjbD), a negative effector of competence in Bacillus subtilis, enhances ClpC-MecA-ComK interaction.
Mol Microbiol: 2002, 44(5);1341-9
[PubMed:12028382] [WorldCat.org] [DOI] (P p)

Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol Microbiol: 2002, 43(5);1331-45
[PubMed:11918817] [WorldCat.org] [DOI] (P p)

Marjan Persuh, Ines Mandic-Mulec, David Dubnau
A MecA paralog, YpbH, binds ClpC, affecting both competence and sporulation.
J Bacteriol: 2002, 184(8);2310-3
[PubMed:11914365] [WorldCat.org] [DOI] (P p)

M M Nakano, F Hajarizadeh, Y Zhu, P Zuber
Loss-of-function mutations in yjbD result in ClpX- and ClpP-independent competence development of Bacillus subtilis.
Mol Microbiol: 2001, 42(2);383-94
[PubMed:11703662] [WorldCat.org] [DOI] (P p)

M Persuh, K Turgay, I Mandic-Mulec, D Dubnau
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch.
Mol Microbiol: 1999, 33(4);886-94
[PubMed:10447896] [WorldCat.org] [DOI] (P p)

M Ogura, L Liu, M Lacelle, M M Nakano, P Zuber
Mutational analysis of ComS: evidence for the interaction of ComS and MecA in the regulation of competence development in Bacillus subtilis.
Mol Microbiol: 1999, 32(4);799-812
[PubMed:10361283] [WorldCat.org] [DOI] (P p)

K Turgay, J Hahn, J Burghoorn, D Dubnau
Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor.
EMBO J: 1998, 17(22);6730-8
[PubMed:9890793] [WorldCat.org] [DOI] (P p)

T Msadek, V Dartois, F Kunst, M L Herbaud, F Denizot, G Rapoport
ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation.
Mol Microbiol: 1998, 27(5);899-914
[PubMed:9535081] [WorldCat.org] [DOI] (P p)

K Turgay, L W Hamoen, G Venema, D Dubnau
Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis.
Genes Dev: 1997, 11(1);119-28
[PubMed:9000055] [WorldCat.org] [DOI] (P p)

J Hahn, J Bylund, M Haines, M Higgins, D Dubnau
Inactivation of mecA prevents recovery from the competent state and interferes with cell division and the partitioning of nucleoids in Bacillus subtilis.
Mol Microbiol: 1995, 18(4);755-67
[PubMed:8817496] [WorldCat.org] [DOI] (P p)

J Hahn, L Kong, D Dubnau
The regulation of competence transcription factor synthesis constitutes a critical control point in the regulation of competence in Bacillus subtilis.
J Bacteriol: 1994, 176(18);5753-61
[PubMed:8083167] [WorldCat.org] [DOI] (P p)

L Kong, D Dubnau
Regulation of competence-specific gene expression by Mec-mediated protein-protein interaction in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1994, 91(13);5793-7
[PubMed:8016067] [WorldCat.org] [DOI] (P p)

T Msadek, F Kunst, G Rapoport
MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a pleiotropic regulator controlling competence gene expression and growth at high temperature.
Proc Natl Acad Sci U S A: 1994, 91(13);5788-92
[PubMed:8016066] [WorldCat.org] [DOI] (P p)

L Kong, K J Siranosian, A D Grossman, D Dubnau
Sequence and properties of mecA, a negative regulator of genetic competence in Bacillus subtilis.
Mol Microbiol: 1993, 9(2);365-73
[PubMed:8412687] [WorldCat.org] [DOI] (P p)

M Roggiani, J Hahn, D Dubnau
Suppression of early competence mutations in Bacillus subtilis by mec mutations.
J Bacteriol: 1990, 172(7);4056-63
[PubMed:2113920] [WorldCat.org] [DOI] (P p)