Difference between revisions of "FruA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fruK]]'', ''[[sipT]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fruK]]'', ''[[sipT]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14400 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14400 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14400 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14400 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14400 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14400 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:fruA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:fruA_context.gif]]

Revision as of 10:05, 14 May 2013

Gene name fruA
Synonyms
Essential no
Product fructose-specific phosphotransferase system, EIIABC
Function fructose uptake and phosphorylation
Gene expression levels in SubtiExpress: fruA
Interactions involving this protein in SubtInteract: FruA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 67 kDa, 5.241
Gene length, protein length 1905 bp, 635 aa
Immediate neighbours fruK, sipT
Sequences Protein DNA DNA_with_flanks
Genetic context
FruA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FruA expression.png




























Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

FruR regulon

The gene

Basic information

  • Locus tag: BSU14400

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed
  • Paralogous protein(s): ManP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in the presence of fructose (FruR) (according to DBTBS)
  • Regulatory mechanism:
    • FruR: transcription repression (according to DBTBS)
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)