Difference between revisions of "CccA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trmK]]'', ''[[sigA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trmK]]'', ''[[sigA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25190 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25190 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25190 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25190 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25190 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25190 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:cccA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:cccA_context.gif]]

Revision as of 10:44, 14 May 2013

  • Description: cytochrome c550

Gene name cccA
Synonyms
Essential no
Product cytochrome c550
Function respiration
Gene expression levels in SubtiExpress: cccA
MW, pI 12 kDa, 5.276
Gene length, protein length 360 bp, 120 aa
Immediate neighbours trmK, sigA
Sequences Protein DNA DNA_with_flanks
Genetic context
CccA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

respiration

This gene is a member of the following regulons

CcpA regulon, AbrB regulon

The gene

Basic information

  • Locus tag: BSU25190

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • CcpA: transcription repression, cre site overlaps -35 region of the promoter PubMed
    • AbrB: transcription repression PubMed
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

V Monedero, G Boël, J Deutscher
Catabolite regulation of the cytochrome c550-encoding Bacillus subtilis cccA gene.
J Mol Microbiol Biotechnol: 2001, 3(3);433-8
[PubMed:11361075] [WorldCat.org] (P p)

P S David, M R Morrison, S L Wong, B C Hill
Expression, purification, and characterization of recombinant forms of membrane-bound cytochrome c-550nm from Bacillus subtilis.
Protein Expr Purif: 1999, 15(1);69-76
[PubMed:10024472] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Physico-chemical characterisation of membrane-bound and water-soluble forms of Bacillus subtilis cytochrome c-550.
Eur J Biochem: 1993, 212(2);499-509
[PubMed:8383048] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis holo-cytochrome c-550 can be synthesised in aerobic Escherichia coli.
FEBS Lett: 1990, 270(1-2);147-51
[PubMed:2171986] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis 13-kilodalton cytochrome c-550 encoded by cccA consists of a membrane-anchor and a heme domain.
J Biol Chem: 1990, 265(23);13939-48
[PubMed:2166045] [WorldCat.org] (P p)