Difference between revisions of "PucH"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yunG]]'', ''[[pucR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yunG]]'', ''[[pucR]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32410 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32410 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:pucH_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pucH_context.gif]]

Revision as of 11:11, 14 May 2013

  • Description: allantoinase

Gene name pucH
Synonyms yunH
Essential no
Product allantoinase
Function purine utilization
Gene expression levels in SubtiExpress: pucH
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 48 kDa, 5.062
Gene length, protein length 1338 bp, 446 aa
Immediate neighbours yunG, pucR
Sequences Protein DNA DNA_with_flanks
Genetic context
PucH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucH expression.png




























Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

  • Locus tag: BSU32410

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-allantoin + H2O = allantoate (according to Swiss-Prot)
  • Protein family: Allantoinase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): PyrC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3HM7 (from Bacillus halodurans, 44% identity, 65% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)