Difference between revisions of "Pgi"
| Line 119: | Line 119: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP508 (spc), available in [[Stülke]] lab | + | * '''Mutant:''' GP508 (spc), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}} |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** pGP398 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab | + | ** pGP398 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab |
| − | * '''lacZ fusion:''' pGP510 (in [[pAC6]]), available in [[Stülke]] lab | + | * '''lacZ fusion:''' pGP510 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
| Line 140: | Line 140: | ||
=References= | =References= | ||
| − | [http://www.ncbi.nlm.nih.gov/pubmed/ | + | [http://www.ncbi.nlm.nih.gov/pubmed/PubMed] |
| − | <pubmed>17218307 19052382 4214896 11489127 4275311 11491085 17218307, </pubmed> | + | <pubmed>17218307 19052382 4214896 23420519 11489127 4275311 11491085 17218307, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:01, 13 July 2013
- Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme
| Gene name | pgi |
| Synonyms | yugL |
| Essential | no |
| Product | glucose-6-phosphate isomerase |
| Function | enzyme in glycolysis / gluconeogenesis |
| Gene expression levels in SubtiExpress: pgi | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
| MW, pI | 50.4 kDa, 4.85 |
| Gene length, protein length | 1353 bp, 451 amino acids |
| Immediate neighbours | yugM, yugK |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31350
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate
- Protein family: GPI family (according to Swiss-Prot) GPI family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- Modification: phosphorylation on Thr-39 PubMed
- Cofactor(s):
- Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed
- Localization:
- cytoplasm (according to Swiss-Prot), cytoplasm
Database entries
- UniProt: P80860
- KEGG entry: [3]
- E.C. number: 5.3.1.9
Additional information
- extensive information on the structure and enzymatic properties of Pgi can be found at Proteopedia
Expression and regulation
- Regulation: constitutively expressed PubMed
- Additional information:
Biological materials
- Mutant: GP508 (spc), available in Jörg Stülke's lab, PubMed
- Expression vector:
- pGP398 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion: pGP510 (in pAC6), available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany homepage
Your additional remarks
References
