Ffh

• Description: signal recognition particle (SRP) component
  
  

Gene name	ffh
Synonyms
Essential	yes PubMed
Product	signal recognition particle (SRP) component
Function	presecretory protein translocation
Gene expression levels in SubtiExpress: ffh
Interactions involving this protein in SubtInteract: Ffh
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	49 kDa, 9.422
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	ylxM, rpsP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15980

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: GTP-binding SRP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SecA-Ffh PubMed
  • Ffh-Scr in the signal recognition particle PubMed
  • Ffh-FtsY in the signal recognition particle PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:
  • 2XXA (complex Ffh-FtsY-Scr RNA, from E. coli) PubMed

• UniProt: P37105

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxM-ffh PubMed

• Expression browser: ffh PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ottilie von Loeffelholz, Kèvin Knoops, Aileen Ariosa, Xin Zhang, Manikandan Karuppasamy, Karine Huard, Guy Schoehn, Imre Berger, Shu-ou Shan, Christiane Schaffitzel
Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex.
Nat Struct Mol Biol: 2013, 20(5);604-10
[PubMed:23563142] [WorldCat.org] [DOI] (I p)

Sandro F Ataide, Nikolaus Schmitz, Kuang Shen, Ailong Ke, Shu-ou Shan, Jennifer A Doudna, Nenad Ban
The crystal structure of the signal recognition particle in complex with its receptor.
Science: 2011, 331(6019);881-6
[PubMed:21330537] [WorldCat.org] [DOI] (I p)

Geeske Zanen, Haike Antelmann, Rob Meima, Jan D H Jongbloed, Marc Kolkman, Michael Hecker, Jan Maarten van Dijl, Wim J Quax
Proteomic dissection of potential signal recognition particle dependence in protein secretion by Bacillus subtilis.
Proteomics: 2006, 6(12);3636-48
[PubMed:16705751] [WorldCat.org] [DOI] (P p)

K Bunai, K Yamada, K Hayashi, K Nakamura, K Yamane
Enhancing effect of Bacillus subtilis Ffh, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitro.
J Biochem: 1999, 125(1);151-9
[PubMed:9880811] [WorldCat.org] [DOI] (P p)

H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318] [WorldCat.org] [DOI] (P p)

K Honda, K Nakamura, M Nishiguchi, K Yamane
Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh.
J Bacteriol: 1993, 175(15);4885-94
[PubMed:8335643] [WorldCat.org] [DOI] (P p)