Difference between revisions of "DegU"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* defect in [[biofilm formation]] {{PubMed|20815827}}, this can be suppressed by DegU-independent expression of [[YuaB]] {{PubMed|21742882}}
 
* defect in [[biofilm formation]] {{PubMed|20815827}}, this can be suppressed by DegU-independent expression of [[YuaB]] {{PubMed|21742882}}
 +
* the mutation suppresses the mucoid phenotype of ''[[motA]]'' or ''[[motB]]'' mutants due to reduced expression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon {{PubMed|24296669}}
 +
 
=== Database entries ===
 
=== Database entries ===
  
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:''' phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity  
 
* '''Modification:''' phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity  
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''  
 
* '''Effectors of protein activity:'''  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=degU_3644607_3645296_-1 degU] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=degU_3644607_3645296_-1 degU] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
 
** ''[[degS]]'': [[SigA]] {{PubMed|1688843}}  
 
** ''[[degS]]'': [[SigA]] {{PubMed|1688843}}  
 
** ''[[degU]]'': [[SigA]] {{PubMed|18502860}}  
 
** ''[[degU]]'': [[SigA]] {{PubMed|18502860}}  
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<pubmed>12471443,,</pubmed>
 
<pubmed>12471443,,</pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 </pubmed>
+
<pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:09, 4 December 2013

  • Description: two-component response regulator, regulation of degradative enzyme and genetic competence, involved in activation of capsule biosynthetic operon expression (together with SwrAA), non-phosphorylated DegU is required for swarming motility PubMed

Gene name degU
Synonyms sacU, iep
Essential no
Product two-component response regulator
Function regulation of degradative enzyme and genetic competence
Gene expression levels in SubtiExpress: degU
Interactions involving this protein in SubtInteract: DegU
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 25 kDa, 5.602
Gene length, protein length 687 bp, 229 aa
Immediate neighbours yviA, degS
Sequences Protein DNA DNA_with_flanks
Genetic context
DegU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DegU expression.png















Categories containing this gene/protein

biofilm formation, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, DegU regulon, TnrA regulon

The DegU regulon

The gene

Basic information

  • Locus tag: BSU35490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activator of degradative enzyme genes (sacB and degQ) and of comK expression
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Robert Belas
When the swimming gets tough, the tough form a biofilm.
Mol Microbiol: 2013, 90(1);1-5
[PubMed:23927648] [WorldCat.org] [DOI] (I p)

Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732] [WorldCat.org] [DOI] (I p)

Ewan J Murray, Taryn B Kiley, Nicola R Stanley-Wall
A pivotal role for the response regulator DegU in controlling multicellular behaviour.
Microbiology (Reading): 2009, 155(Pt 1);1-8
[PubMed:19118340] [WorldCat.org] [DOI] (P p)


The DegU regulon

U Mäder, H Antelmann, T Buder, M K Dahl, M Hecker, G Homuth
Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics.
Mol Genet Genomics: 2002, 268(4);455-67
[PubMed:12471443] [WorldCat.org] [DOI] (P p)

Original Publications