Difference between revisions of "PepF"

From SubtiWiki
Jump to: navigation, search
Line 120: Line 120:
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2180 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2347 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:27, 17 April 2014

  • Description: oligoendopeptidase, inhibits sporulation upon overexpression

Gene name pepF
Synonyms yjbG
Essential no
Product oligoendopeptidase
Function protein degradation
Gene expression levels in SubtiExpress: pepF
MW, pI 69 kDa, 5.131
Gene length, protein length 1827 bp, 609 aa
Immediate neighbours coiA, yizD
Sequences Protein DNA DNA_with_flanks
Genetic context
YjbG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PepF expression.png
























Categories containing this gene/protein

phosphorelay

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase M3B family (according to Swiss-Prot)
  • Paralogous protein(s): YusX

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2180 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2347 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kyoko Kanamaru, Sophie Stephenson, Marta Perego
Overexpression of the PepF oligopeptidase inhibits sporulation initiation in Bacillus subtilis.
J Bacteriol: 2002, 184(1);43-50
[PubMed:11741842] [WorldCat.org] [DOI] (P p)