GltX

• Description: glutamyl-tRNA synthetase, universally conserved protein
  
  

Gene name	gltX
Synonyms
Essential	yes PubMed
Product	glutamyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: gltX
Interactions involving this protein in SubtInteract: GltX
Metabolic function and regulation of this protein in SubtiPathways: gltX
MW, pI	55 kDa, 5.021
Gene length, protein length	1449 bp, 483 aa
Immediate neighbours	ispF, cysE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU00920

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00920

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU00920

• Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed

• UniProt: P22250

• KEGG entry: [3]

• E.C. number: 6.1.1.17

Additional information

universally conserved protein

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Expression browser: gltX PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression transiently increases in the forespore PubMed

• Regulatory mechanism:

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1736 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 5280 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alex Rosenberg, Lior Sinai, Yoav Smith, Sigal Ben-Yehuda
Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.
PLoS One: 2012, 7(7);e41921
[PubMed:22848659] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)