Register here for the SubtiWiki workshop at the 22nd International Conference on Bacilli & Gram-Positive Bacteria in Bloomington in June 2024 (conference website)!
We will show you how to use the newest features of SubtiWiki's next release, such as visualization of conserved gene arrangements and dedicated metabolite pages! You can get a sneak-peek here: corewiki.uni-goettingen.de
mecA
168
buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] and [protein|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS] to the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] degradation machine (in log phase), inhibits the transcriptional activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P by direct interaction
Molecular weight
25.62 kDa
function
control of [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] degradation, regulation of competence
Genomic Context
This gene is a member of the following
regulons
Gene
Coordinates
1,229,068 1,229,724
Phenotypes of a mutant
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
Advertisement
The protein
Catalyzed reaction/ biological activity
recruits protein for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] [pubmed|12598648]
inhibits transcription activation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P (in complex with [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]) [pubmed|29446505]
Protein family
mecA family (with [protein|CE1C46944E242006383BA77B2C4E09045CB3B93F|ypbH], according to UniProt)
[wiki|Domains]
N-terminal domain (1-120): recruitment of protein substrates [Pubmed|19801546]
C-terminal domain (121-218): facilitates assembly of the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] oligomer and acts as a degradation tag [Pubmed|19801546]
Structure
[PDB|3PXG] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [pubmed|21368759]
[PDB|3J3U] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [Pubmed|23595989]
[PDB|3JTP](C-terminal domain) [Pubmed|19801546]
[AF|P37958]
Paralogous protein(s)
[protein|CE1C46944E242006383BA77B2C4E09045CB3B93F|ypbH]
Expression and Regulation
Operons
genes
[gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]
description
[Pubmed|8412687]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8412687], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab
[gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]
2024-06-09 03:27:29
ghost
52
a7eff32f2142ccd5507e9841a00a021df33d8346
9393514154CA17D711EA90973907CF0CBFF6C1FF
Biological materials
Mutant
GP813 (spc), GP814 (aphA3) both available in [wiki|Jörg Stülke]'s lab
BKE11520 ([gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
BKK11520 ([gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
23375660,23479438,19609260,34438398 Original Publications
11914365,9000055,17560370,19361434,9890793,2113920,8817496,8412687,11918817,10447896,12028382,8016067,10361283,11703662,9535081,19767395,19801546,8016066,19202088,8083167,21368759,23595989,16525504,12598648,21435029,29165246,29446505,35638827