DprA

• Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex
  
  

Gene name	dprA
Synonyms	smf
Essential	no
Product	recombination mediator protein
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI	32 kDa, 9.755
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	sucD, topA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU16110

Phenotypes of a mutant

• • strongly impaired genetic recombination PubMed

Database entries

• BsubCyc: BSU16110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds ssDNA, promotes displacement of SsbA and SsbB from ssDNA PubMed
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed

• Protein family: smf family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • DprA-RecA PubMed
  • DprA-DprA PubMed
  • DprA-ComFA PubMed
  • SsbB-DprA PubMed

• Localization:

Database entries

• BsubCyc: BSU16110

• Structure:

• UniProt: P39813

• KEGG entry: [3]

Expression and regulation

• Operon: dprA (according to DBTBS)

• Expression browser: dprA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger genetic competence (ComK) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 57 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Tribhuwan Yadav, Begoña Carrasco, James Hejna, Yuki Suzuki, Kunio Takeyasu, Juan C Alonso
Bacillus subtilis DprA recruits RecA onto single-stranded DNA and mediates annealing of complementary strands coated by SsbB and SsbA.
J Biol Chem: 2013, 288(31);22437-50
[PubMed:23779106] [WorldCat.org] [DOI] (I p)

Nicolas Mirouze, Mathieu A Bergé, Anne-Lise Soulet, Isabelle Mortier-Barrière, Yves Quentin, Gwennaele Fichant, Chantal Granadel, Marie-Françoise Noirot-Gros, Philippe Noirot, Patrice Polard, Bernard Martin, Jean-Pierre Claverys
Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.
Proc Natl Acad Sci U S A: 2013, 110(11);E1035-44
[PubMed:23440217] [WorldCat.org] [DOI] (I p)

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Serkalem Tadesse, Peter L Graumann
DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells.
BMC Microbiol: 2007, 7;105
[PubMed:18045469] [WorldCat.org] [DOI] (I e)

Isabelle Mortier-Barrière, Marion Velten, Pauline Dupaigne, Nicolas Mirouze, Olivier Piétrement, Stephen McGovern, Gwennaele Fichant, Bernard Martin, Philippe Noirot, Eric Le Cam, Patrice Polard, Jean-Pierre Claverys
A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA.
Cell: 2007, 130(5);824-36
[PubMed:17803906] [WorldCat.org] [DOI] (P p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Janet C Lindow, Masayoshi Kuwano, Shigeki Moriya, Alan D Grossman
Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein.
Mol Microbiol: 2002, 46(4);997-1009
[PubMed:12421306] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)