Register here for the SubtiWiki workshop at the 22nd International Conference on Bacilli & Gram-Positive Bacteria in Bloomington in June 2024 (conference website)!
We will show you how to use the newest features of SubtiWiki's next release, such as visualization of conserved gene arrangements and dedicated metabolite pages! You can get a sneak-peek here: corewiki.uni-goettingen.de
dprA
168
conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]; facilitates the displacement of both SSBs ([protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]), increases [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation
Molecular weight
32.78 kDa
function
[category|SW.3.1.5|DNA repair/ recombination]
product
recombination mediator protein
Genomic Context
This gene is a member of the following
regulons
Gene
Coordinates
1,682,580 → 1,683,473
Phenotypes of a mutant
strongly impaired genetic recombination [Pubmed|23779106]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
loss of plasmid transformation [pubmed|30050509]
Advertisement
The protein
Catalyzed reaction/ biological activity
binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] from ssDNA [Pubmed|23779106]
provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]) [Pubmed|22373918]
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] onto any fragmented linear SPP1 ssDNA [pubmed|31876108]
anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
Protein family
DprA/Smf family (single member, according to UniProt)
Structure
[PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]
[AF|P39813]
[wiki|Localization]
transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]
Expression and Regulation
Operons
genes
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
regulation
repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
expression is heterogeneous [pubmed|29809139]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Open in new tab
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]→[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
2024-06-07 20:52:40
Jstuelk
65
167fd8fae94fab138af8eabe6101bc58adfb0921
2BC79D48E345B722DDAE396E448CF1DB3AAB049C
additional information
expressed under conditions that trigger genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]) [Pubmed|11948146]
Biological materials
Mutant
BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16110 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Reviews
Original Publications
17630974,17803906,12421306,18045469,11948146,22373918,23779106,23440217,25138221,26786319,28618091,22904190,28911099,30050509,31876108,33264457