Difference between revisions of "RnhB"

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(Reviews)
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* '''Mutant:''' BP423 (''ΔrnhB::aphA3'') available in [[Fabian Commichau]]'s lab
 
* '''Mutant:''' BP423 (''ΔrnhB::aphA3'') available in [[Fabian Commichau]]'s lab
  
* '''Expression vector:'''
+
* '''Expression vector:''' ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in [[Fabian Commichau]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
Line 133: Line 133:
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Fabian Commichau]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 13:23, 7 October 2014

  • Description: RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication

Gene name rnhB
Synonyms rnh
Essential no
Product Mn2+-dependent RNase HII
Function endonucleolytic cleavage of RNA in
RNA-DNA hybrid molecules
Gene expression levels in SubtiExpress: rnhB
MW, pI 28 kDa, 5.518
Gene length, protein length 765 bp, 255 aa
Immediate neighbours rbgA, ylqG
Sequences Protein DNA DNA_with_flanks
Genetic context
RnhB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RnhB expression.png















Categories containing this gene/protein

DNA replication, Rnases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
    • removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed
  • Protein family: RNase HII family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number: 3.1.26.4.

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector: ** for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Karolina A Majorek, Stanislaw Dunin-Horkawicz, Kamil Steczkiewicz, Anna Muszewska, Marcin Nowotny, Krzysztof Ginalski, Janusz M Bujnicki
The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification.
Nucleic Acids Res: 2014, 42(7);4160-79
[PubMed:24464998] [WorldCat.org] [DOI] (I p)

Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197] [WorldCat.org] [DOI] (I p)


Original publications

Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084] [WorldCat.org] [DOI] (I p)

Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985] [WorldCat.org] [DOI] (I p)

Mitsuru Haruki, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya
Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII.
FEBS Lett: 2002, 531(2);204-8
[PubMed:12417313] [WorldCat.org] [DOI] (P p)

M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689] [WorldCat.org] [DOI] (P p)

N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800] [WorldCat.org] [DOI] (P p)