Difference between revisions of "DegU"
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | ** transcription activator (see [[DegU regulon]]) | + | ** transcription activator and repressor (see [[DegU regulon]]) |
| + | ** DegU-P represses expression of the fla/che operon in the absence of [[SwrA]], and switches to an activator of the operon in the presence of [[SwrA]] {{PubMed|24386445}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 103: | Line 104: | ||
** [[DegU]]-[[RapG]] {{PubMed|12950930}} | ** [[DegU]]-[[RapG]] {{PubMed|12950930}} | ||
** [[DegS]]-[[DegU]] | ** [[DegS]]-[[DegU]] | ||
| − | ** [[SwrA]]-[[DegU]] (N-terminal domain) {{PubMed|22496484}} | + | ** [[SwrA]]-[[DegU]]-P (N-terminal domain) {{PubMed|24386445,22496484}} |
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot) | * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot) | ||
| Line 177: | Line 178: | ||
<pubmed>12471443,,</pubmed> | <pubmed>12471443,,</pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669 122328658 23888912 24149708 24317403 25431404 25433860 </pubmed> | + | <pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669 122328658 23888912 24149708 24317403 25431404 25433860 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:45, 29 December 2014
- Description: two-component response regulator, regulation of degradative enzyme expression, genetic competence, biofilm formation, capsule biosynthesis (together with SwrAA), non-phosphorylated DegU is required for swarming motility
| Gene name | degU |
| Synonyms | sacU, iep |
| Essential | no |
| Product | two-component response regulator |
| Function | regulation of degradative enzymes, genetic competence,
biofilm formation and capsule synthesis |
| Gene expression levels in SubtiExpress: degU | |
| Interactions involving this protein in SubtInteract: DegU | |
| Metabolic function and regulation of this protein in SubtiPathways: DegU | |
| MW, pI | 25 kDa, 5.602 |
| Gene length, protein length | 687 bp, 229 aa |
| Immediate neighbours | yviA, degS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biofilm formation, transcription factors and their control, phosphoproteins
This gene is a member of the following regulons
CcpA regulon, DegU regulon, SinR regulon, TnrA regulon
The DegU regulon
The gene
Basic information
- Locus tag: BSU35490
Phenotypes of a mutant
- defect in biofilm formation PubMed, this can be suppressed by DegU-independent expression of BslA PubMed
- the mutation suppresses the mucoid phenotype of motA or motB mutants due to reduced expression of the capB-capC-capA-capE operon PubMed
Database entries
- BsubCyc: BSU35490
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- transcription activator and repressor (see DegU regulon)
- DegU-P represses expression of the fla/che operon in the absence of SwrA, and switches to an activator of the operon in the presence of SwrA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU35490
- Structure:
- UniProt: P13800
- KEGG entry: [3]
- E.C. number:
Additional information
- DegU-P is degraded by ClpC-ClpP PubMed
- accumulation of DegU-P results in decreased transcription of the epsA-epsO and tapA-sipW-tasA operons due to increased levels of Spo0A-P PubMed
Expression and regulation
- Regulatory mechanism:
- Additional information:
- DegU-P is degraded by ClpC-ClpP PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 1627 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 2454 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2251 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1461 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1432 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
The DegU regulon
U Mäder, H Antelmann, T Buder, M K Dahl, M Hecker, G Homuth
Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics.
Mol Genet Genomics: 2002, 268(4);455-67
[PubMed:12471443]
[WorldCat.org]
[DOI]
(P p)
Original Publications
