Difference between revisions of "BceB"

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* '''Description:''' [[ABC transporter]] (permease) for the export of bacitracin, plectasin, mersacidin and actagardine <br/><br/>
+
* '''Description:''' [[ABC transporter]] (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** binds bacitracin, K<sup>D</sup>: 60 nM {{PubMed|25118291}}
 +
** inhibits [[BceS]] autophosphorylation activity in the absence of bacitracin {{PubMed|25118291}}
  
 
* '''Protein family:''' ABC-4 integral membrane protein family (according to Swiss-Prot)
 
* '''Protein family:''' ABC-4 integral membrane protein family (according to Swiss-Prot)
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[BceA]]-[[BceB]] {{PubMed|23687272}}
+
** [[BceA]]-[[BceB]] {{PubMed|25118291,23687272}}
** [[BceB]]-[[BceS]] {{PubMed|23687272,21283517}}
+
** [[BceB]]-[[BceS]], in the absence of bacitracin {{PubMed|25118291,23687272,21283517}}
  
 
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
 
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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=References=
 
=References=
<pubmed>18394148,17905982,12890034,14651641,10092453, 14612242 21283517, 20606066 21078927 23687272 24806199</pubmed>
+
<pubmed>18394148,17905982,12890034,14651641,10092453, 14612242 21283517, 20606066 21078927 23687272 25118291,24806199</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:00, 26 April 2015

  • Description: ABC transporter (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system

Gene name bceB
Synonyms ytsD
Essential no
Product bacitracin ABC transporter (permease)
Function export of toxic peptides
Gene expression levels in SubtiExpress: bceB
Interactions involving this protein in SubtInteract: BceB
MW, pI 72 kDa, 9.79
Gene length, protein length 1938 bp, 646 aa
Immediate neighbours yttA, bceA
Sequences Protein DNA DNA_with_flanks
Genetic context
YtsD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BceB expression.png















Categories containing this gene/protein

ABC transporters, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

BceR regulon

The gene

Basic information

  • Locus tag: BSU30370

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds bacitracin, KD: 60 nM PubMed
    • inhibits BceS autophosphorylation activity in the absence of bacitracin PubMed
  • Protein family: ABC-4 integral membrane protein family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in the presence of bacitracin, plectasin, mersacidin and actagardine (BceR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sebastian Dintner, Ralf Heermann, Chong Fang, Kirsten Jung, Susanne Gebhard
A sensory complex consisting of an ATP-binding cassette transporter and a two-component regulatory system controls bacitracin resistance in Bacillus subtilis.
J Biol Chem: 2014, 289(40);27899-910
[PubMed:25118291] [WorldCat.org] [DOI] (I p)

Anthony W Kingston, Heng Zhao, Gregory M Cook, John D Helmann
Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter.
Mol Microbiol: 2014, 93(1);37-49
[PubMed:24806199] [WorldCat.org] [DOI] (I p)

Felix Kallenberg, Sebastian Dintner, Roland Schmitz, Susanne Gebhard
Identification of regions important for resistance and signalling within the antimicrobial peptide transporter BceAB of Bacillus subtilis.
J Bacteriol: 2013, 195(14);3287-97
[PubMed:23687272] [WorldCat.org] [DOI] (I p)

Stéphanie Coumes-Florens, Céline Brochier-Armanet, Annick Guiseppi, François Denizot, Maryline Foglino
A new highly conserved antibiotic sensing/resistance pathway in firmicutes involves an ABC transporter interplaying with a signal transduction system.
PLoS One: 2011, 6(1);e15951
[PubMed:21283517] [WorldCat.org] [DOI] (I e)

Anna Staroń, Dora Elisabeth Finkeisen, Thorsten Mascher
Peptide antibiotic sensing and detoxification modules of Bacillus subtilis.
Antimicrob Agents Chemother: 2011, 55(2);515-25
[PubMed:21078927] [WorldCat.org] [DOI] (I p)

Jing Ouyang, Xiao-Lin Tian, Jennifer Versey, Alexander Wishart, Yung-Hua Li
The BceABRS four-component system regulates the bacitracin-induced cell envelope stress response in Streptococcus mutans.
Antimicrob Agents Chemother: 2010, 54(9);3895-906
[PubMed:20606066] [WorldCat.org] [DOI] (I p)

Eva Rietkötter, Diana Hoyer, Thorsten Mascher
Bacitracin sensing in Bacillus subtilis.
Mol Microbiol: 2008, 68(3);768-85
[PubMed:18394148] [WorldCat.org] [DOI] (I p)

Remi Bernard, Annick Guiseppi, Marc Chippaux, Maryline Foglino, François Denizot
Resistance to bacitracin in Bacillus subtilis: unexpected requirement of the BceAB ABC transporter in the control of expression of its own structural genes.
J Bacteriol: 2007, 189(23);8636-42
[PubMed:17905982] [WorldCat.org] [DOI] (I p)

Thorsten Mascher, Neil G Margulis, Tao Wang, Rick W Ye, John D Helmann
Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon.
Mol Microbiol: 2003, 50(5);1591-604
[PubMed:14651641] [WorldCat.org] [DOI] (P p)

Remi Bernard, Pascale Joseph, Annick Guiseppi, Marc Chippaux, François Denizot
YtsCD and YwoA, two independent systems that confer bacitracin resistance to Bacillus subtilis.
FEMS Microbiol Lett: 2003, 228(1);93-7
[PubMed:14612242] [WorldCat.org] [DOI] (P p)

Reiko Ohki, Giyanto, Kozue Tateno, Waka Masuyama, Shigeki Moriya, Kazuo Kobayashi, Naotake Ogasawara
The BceRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis.
Mol Microbiol: 2003, 49(4);1135-44
[PubMed:12890034] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)