ConE

• Description: required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer
  
  

Gene name	conE
Synonyms	yddE
Essential	no
Product	part of the type IV secretion system for DNA transfer
Function	conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: conE
MW, pI	96 kDa, 5.511
Gene length, protein length	2493 bp, 831 aa
Immediate neighbours	conD, yddF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

mobile genetic elements, membrane proteins

The gene

Basic information

• Locus tag: BSU04940

Phenotypes of a mutant

• Defective for conjugal transfer of ICEBs1

Database entries

• BsubCyc: BSU04940

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: required for conjugative transfer of ICEBs1, putative ATPase PubMed

• Protein family: HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • peripheral membrane protein (via ConB), located at the cell poles PubMed

Database entries

• BsubCyc: BSU04940

• Structure:

• UniProt: P96642

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: conE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Melanie Barker Berkmen, Suffolk University, Boston, MA Homepage

References

Cori T Leonetti, Matt A Hamada, Stephanie J Laurer, Matthew P Broulidakis, Kyle J Swerdlow, Catherine A Lee, Alan D Grossman, Melanie B Berkmen
Critical Components of the Conjugation Machinery of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2015, 197(15);2558-67
[PubMed:26013486] [WorldCat.org] [DOI] (I p)

Melanie B Berkmen, Catherine A Lee, Emma-Kate Loveday, Alan D Grossman
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2010, 192(1);38-45
[PubMed:19734305] [WorldCat.org] [DOI] (I p)