Difference between revisions of "RpoC"

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(Original publications)
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** [[SigF]]-([[RpoB]]-[[RpoC]]), [[SigG]]-([[RpoB]]-[[RpoC]])
 
** [[SigF]]-([[RpoB]]-[[RpoC]]), [[SigG]]-([[RpoB]]-[[RpoC]])
 
** [[SigH]]-([[RpoB]]-[[RpoC]]), [[SigI]]-([[RpoB]]-[[RpoC]])
 
** [[SigH]]-([[RpoB]]-[[RpoC]]), [[SigI]]-([[RpoB]]-[[RpoC]])
** [[SigK]]-([[RpoB]]-[[RpoC]]), [[SigL]]-([[RpoB]]-[[RpoC]])
+
** [[SigK]]-([[RpoB]]-[[RpoC]])
 +
** [[SigL]]-([[RpoB]]-[[RpoC]]-[[RpoA]]) {{PubMed|26293966}}
 
** [[SigM]]-([[RpoB]]-[[RpoC]]), [[SigV]]-([[RpoB]]-[[RpoC]])
 
** [[SigM]]-([[RpoB]]-[[RpoC]]), [[SigV]]-([[RpoB]]-[[RpoC]])
 
** [[SigW]]-([[RpoB]]-[[RpoC]]), [[SigX]]-([[RpoB]]-[[RpoC]])
 
** [[SigW]]-([[RpoB]]-[[RpoC]]), [[SigX]]-([[RpoB]]-[[RpoC]])
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<pubmed> 22210308 24763425 </pubmed>
 
<pubmed> 22210308 24763425 </pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>7657605,12486038,10675340,7592585, 19735077,18763711, 19680289 22517742 23070162,20817769 24572018 25092033 24491578 26243774</pubmed>
+
<pubmed>7657605,12486038,10675340,7592585, 19735077,18763711, 19680289 22517742 23070162,20817769 24572018 25092033 24491578 26243774 26293966</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:28, 30 August 2015

Gene name rpoC
Synonyms
Essential yes PubMed
Product RNA polymerase beta' subunit
Function transcription
Gene expression levels in SubtiExpress: rpoC
Interactions involving this protein in SubtInteract: RpoC
MW, pI 133 kDa, 8.863
Gene length, protein length 3597 bp, 1199 aa
Immediate neighbours rpoB, ybxF
Sequences Protein DNA DNA_with_flanks
Genetic context
RpoC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RpoC expression.png















Categories containing this gene/protein

transcription, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01080

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • mutations in mtrB, sigB, rpoB, and rpoC allow B. subtilis to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)
  • Protein family: RNA polymerase beta' chain family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-335 and Arg-803 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2487 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 8264 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Nikolay Zenkin
Multiple personalities of the RNA polymerase active centre.
Microbiology (Reading): 2014, 160(Pt 7);1316-1320
[PubMed:24763425] [WorldCat.org] [DOI] (I p)

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)

Original publications