Difference between revisions of "SigL"

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|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sigma-54 factor (sigma-L)
 
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sigma-54 factor (sigma-L)
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin, and fructose, required for cold adaptation
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49,5 kDa, 7.79
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49,5 kDa, 7.79
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__TOC__
 
__TOC__
  
<br/><br/>
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=The gene=
 
=The gene=

Revision as of 13:20, 6 April 2009

  • Description: Sigma subunit of the RNA polymerase, Sigma-54, Sigma L

Gene name sigL
Synonyms
Essential no
Product RNA polymerase sigma-54 factor (sigma-L)
Function utilization of arginin, acetoin and fructose,
required for cold adaptation
MW, pI 49,5 kDa, 7.79
Gene length, protein length 1308 bp, 436 amino acids
Immediate neighbours yvfG, yvfH
Gene sequence (+200bp) Protein sequence
Genetic context
SigL context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates: 3511532 - 3512839

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
  • Protein family: sigma-54 factor family
  • Paralogous protein(s):

Genes/ operons controlled by SigL

acoA-acoB-acoC-acoL, rocA-rocB-rocC, rocD-rocE-rocF, rocG, levD-levE-levF-levG-sacC, ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA binding domain (H-T-H motif) (324–343)
    • pron box domain (413–421)
    • 3 x Compositional bias domain (6–21),(32–53),(112–136)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry: [3]
  • KEGG entry: [4]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by glucose (CcpA) , neg. regulated by CcpA PubMed
  • Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock
  • Additional information:

Biological materials

  • Mutant: GP146 (aphA3), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

  1. Ali, N. O., Bignon, J., Rapoport, G., and Débarbouillé, M. (2001) Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis. J. Bacteriol. 183, 2497-2504. PubMed
  2. Belitsky BR, Sonenshein AL (1998) Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J Bacteriol 180:6298-6305. PubMed
  3. Choi SK, Saier MH Jr: (2005) Regulation of sigL expression by the catabolite control protein CcpA involves a roadblock mechanism in Bacillus subtilis: potential connection between carbon and nitrogen metabolism. J Bacteriol , 187:6856-6861. PubMed
  4. Débarbouillé M, Martin-Verstraete I, Kunst F, Rapoport G: (1991) The Bacillus subtilis sigL gene encodes an equivalent of (Sigma)54 from Gram-negative bacteria. Proc Natl Acad Sci USA , 88:9092-9096. PubMed
  5. Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. PubMed
  6. Wiegeshoff et al. (2006) Sigma L is important for cold shock adaptation of Bacillus subtilis. J Bacteriol , 188: 3130-3133. PubMed