Difference between revisions of "CitZ"

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* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
  
* '''Antibody:'''
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* '''Antibody:''' available in [[Linc Sonenshein]] lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 18:43, 28 April 2009

  • Description: citrate synthase

Gene name citZ
Synonyms citA2
Essential no
Product citrate synthase II
Function TCA cycle
MW, pI 41 kDa, 5.451
Gene length, protein length 1116 bp, 372 aa
Immediate neighbours ytwI, icd
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CitZ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

glutamate auxotrophy and a defect in sporulation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acetyl-CoA + oxaloacetate ---> citrate, the reaction is irreversible
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-284 PubMed
  • Cofactor(s):
  • Effectors of protein activity: inhibited by ATP PubMed
  • Interactions:
  • Localization:

Database entries

  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number: 4.1.3.7

Additional information

Expression and regulation

  • Regulation: repressed by glucose (6.7-fold) (CcpA) PubMed, catabolite repression (CcpA), repression by glucose + glutamate (CcpC) PubMed, repression under anaerobic conditions PubMed
  • Regulatory mechanism: CcpA: transcription repression, CcpA: transcription repression, CcpC: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant: GP678 (erm), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
  3. Kim HJ, Roux A, Sonenshein AL (2002b) Direct and indirect roles of CcpA in regulation of Bacillus subtilis Krebs cycle genes. Mol Microbiol 45:179-190. PubMed
  4. Nakano MM, Zuber P, Sonenshein AL (1998) Anaerobic regulation of Bacillus subtilis Krebs cycle genes. J Bacteriol. 180: 3304-3311. PubMed
  5. Jin S, Sonenshein AL (1994) Identification of two distinct Bacillus subtilis citrate synthase genes J Bacteriol. 176: 4669-4679. PubMed
  6. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed