Difference between revisions of "LevE"

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Line 82: Line 82:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P26380 P26380]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P26380 P26380]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27060]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27060 BSU27060]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69]

Revision as of 23:13, 13 May 2009

  • Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component

Gene name levE
Synonyms sacL
Essential no
Product trigger enzyme: fructose-specific phosphotransferase system, EIIB component

fructose-specific enzyme IIB (EC 2.7.1.69)

Function fructose uptake and phosphorylation, control of LevR activity
MW, pI 17 kDa, 9.444
Gene length, protein length 486 bp, 162 aa
Immediate neighbours levF, levD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LevE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  1. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
  2. Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the Bacillus subtilis transcriptional activator LevR. Mol. Microbiol. 28: 293-303. PubMed
  3. Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T. 1998. Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis. J. Mol. Biol. 276:591-602. PubMed
  4. Seip, S., R. Lanz, R. Gutknecht, K. Flükiger, and B. Erni. 1997. The fructose transporter of Bacillus subtilis encoded by the lev operon. Backbone assignment and secondary structure of the IIBLev subunit. Eur. J. Biochem. 243: 306-314. PubMed
  5. Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. PubMed
  6. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed