Difference between revisions of "DegS"

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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' autophosphorylation, autodephosphorylation, phosphorylation of [[DegU]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 64: Line 64:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:''' phosphorylation on Ser-76 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
+
** aa  12 ... 170: unknown DegS domain
 +
** aa 180 ... 248: HisKA_3 domain (autophosphorylation)
 +
** aa 289 ... 384: HATPase_c
 +
 
 +
* '''Modification:''' phosphorylation on Ser-76 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], autophosphorylation on His-189
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:''' [[DegQ]]
  
* '''Interactions:''' [[DegS]]-[[DegU]]
+
* '''Interactions:''' [[DegS]]-[[DegU]], [[DegS]]-[[DegQ]]
  
 
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

Revision as of 09:06, 21 May 2009

  • Description: two-component sensor kinase for exoenzyme and competence regulation

Gene name degS
Synonyms sacU
Essential no
Product two-component sensor kinase (NarL family)
Function regulation of degradative enzyme and genetic competence
MW, pI 44 kDa, 5.988
Gene length, protein length 1155 bp, 385 aa
Immediate neighbours degU, yvyE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DegS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, autodephosphorylation, phosphorylation of DegU on Asp-56 PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • aa 12 ... 170: unknown DegS domain
    • aa 180 ... 248: HisKA_3 domain (autophosphorylation)
    • aa 289 ... 384: HATPase_c
  • Modification: phosphorylation on Ser-76 PubMed, autophosphorylation on His-189
  • Cofactor(s):
  • Effectors of protein activity: DegQ
  • Localization: Cytoplasm (Homogeneous) PubMed

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced in the presence of ammonium (TnrA)
  • Regulatory mechanism: TnrA: repression, DegU: positive autoregulation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Tarek Msadek, Institut Pasteur, Paris, France

Your additional remarks

References

  1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
  2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed