Difference between revisions of "NrdF"

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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H<sub>2</sub>O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  
 
* '''Protein family:''' ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
 
* '''Protein family:''' ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)

Revision as of 21:33, 23 May 2009

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdF
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
MW, pI 38 kDa, 4.66
Gene length, protein length 987 bp, 329 aa
Immediate neighbours nrdE, ymaB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NrdF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  • Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
  • Paralogous protein(s): YosP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: distinct promoters for aerobic and anaerobic expression PubMed, anaerobic expression requires ResD PubMed
  • Regulatory mechanism: ResD: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed