Difference between revisions of "BofA"
| Line 37: | Line 37: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU00230 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 82: | Line 82: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P24282 P24282] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P24282 P24282] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00230] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 13:36, 3 June 2009
- Description: inhibition of the pro-sigma-K processing machinery
| Gene name | bofA |
| Synonyms | |
| Essential | no |
| Product | inhibitor of pro-SigK processing |
| Function | control of SigK activation |
| MW, pI | 8 kDa, 9.32 |
| Gene length, protein length | 261 bp, 87 aa |
| Immediate neighbours | yaaL, rrnA-16S |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU00230
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: forespore outer membrane (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P24282
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: bofA PubMed
- Regulation: expressed during sporulation PubMed
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Simon Cutting, London, UK homepage
Your additional remarks
References
- Dong & Cutting (2004) The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis.J. Biol. Chem. 279: 43468-43478. PubMed
- Ireton & Grossman (1992 ) Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis. J. Bacteriol. 174: 3185-3195. PubMed
