Difference between revisions of "FadN"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU32840
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32178 O32178]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32178 O32178]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32840 BSU32840]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32840]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.35 1.1.1.35]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.35 1.1.1.35]

Revision as of 13:46, 3 June 2009

  • Description: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

Gene name fadN
Synonyms yusL
Essential no
Product 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
Function fatty acid degradation
MW, pI 89 kDa, 6.53
Gene length, protein length 2445 bp, 815 aa
Immediate neighbours fadA, fadM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YusL context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU32840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH (according to Swiss-Prot)
  • Protein family: 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by glucose (4.5-fold) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Matsuoka H, Hirooka K, Fujita Y. (2007) Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation. J Biol Chem. Feb 23;282(8): 5180-94. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed