Difference between revisions of "Sandbox"

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−
* '''Description:''' general stress protein <br/><br/>
+
* '''Description:''' forespore-specific protein, inhibitor of [[SigG]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''csbX''
+
|''csfB''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaM ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || inhibitor of [[SigG]]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigG]] activity
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 8.818  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 7.671  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1305 bp, 435 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 192 bp, 64 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bofC]]'', ''[[yrbE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rrnA-5S]]'', ''[[xpaC]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14736&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11800&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|-
+
|colspan="2" | '''Genetic context''' <br/> [[Image:csfB_context.gif]]
−
|-
 
−
|colspan="2" | '''Genetic context''' <br/> [[Image:csbX_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU27760
+
* '''Locus tag:''' BSU00240
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 +
premature activation of [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csbX-bofC.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csfB.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11922]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10088]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 53:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' inhibitor of [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/19497328 PubMed2]
 +
 
  
 
* '''Protein family:'''
 
* '''Protein family:'''
  
−
* '''Paralogous protein(s):''' [[YoaB]]
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 72: Line 72:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''Interactions:''' [[CsfB]]-[[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19497328 PubMed]
  
−
* '''Localization:''' forespore intermembrane space (according to Swiss-Prot)
+
* '''Localization:''' forespore
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05390 O05390]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37534 P37534]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27760]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00240]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''csfB''
  
−
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]  
+
* '''[[Sigma factor]]:''' [[SigF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
  
−
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 120:
 
=References=
 
=References=
  
−
<pubmed>11544224, </pubmed>
+
<pubmed>16497325 17921305, </pubmed>
−
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
+
# Wang et al. (2006) The forespore line of gene expression in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''358:''' 16-37. [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
 +
# Chary et al. (2007) Expression of the sigmaF-directed ''csfB'' locus prevents premature appearance of sigmaG activity during sporulation of ''Bacillus subtilis''.''J. Bacteriol.'' '''189:''' 8754-8757. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:13, 8 June 2009

  • Description: forespore-specific protein, inhibitor of SigG

Gene name csfB
Synonyms yaaM
Essential no
Product inhibitor of SigG
Function control of SigG activity
MW, pI 7 kDa, 7.671
Gene length, protein length 192 bp, 64 aa
Immediate neighbours rrnA-5S, xpaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsfB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00240

Phenotypes of a mutant

premature activation of SigG PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution


  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: forespore

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: csfB
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Wang et al. (2006) The forespore line of gene expression in Bacillus subtilis. J. Mol. Biol. 358: 16-37. PubMed
  2. Chary et al. (2007) Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis.J. Bacteriol. 189: 8754-8757. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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