Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
−
* '''Description:''' phosphoadenosine phosphosulfate sulfotransferase <br/><br/>
+
* '''Description:''' trigger enzyme: cysteine synthetase A and control of [[CymR]] activity<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''cysH''
+
|''cysK''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphoadenosine phosphosulfate sulfotransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || cysteine synthetase A
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || sulfate reduction
+
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of cysteine, control of [[CymR]] activity
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 5.462  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.492  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 699 bp, 233 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 924 bp, 308 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pyrE]]'', ''[[cysP]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yacD]]'', ''[[pabB]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13431&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11849&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:cysH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:cysK_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU15570
+
* '''Locus tag:''' BSU00730
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
constitutive expression of the ''[[cysH]]-[[cysP]]-[[sat]]-[[cysC]]-[[ylnD]]-[[ylnE]]-[[ylnF]]'' operon, auxotrophic for cysteine
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysK.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11930]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10136]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' O(3)-acetyl-L-serine + H<sub>2</sub>S = L-cysteine + acetate (according to Swiss-Prot)  
  
−
* '''Protein family:''' CysH subfamily (according to Swiss-Prot)
+
* '''Protein family:''' cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
  
−
* '''Paralogous protein(s):''' [[YitB]]
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 70: Line 72:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''Interactions:''' [[CymR]]-[[CysK]], the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
  
−
* '''Localization:''' cytoplasm (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 78: Line 80:
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94498 P94498]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37887 P37887]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15570]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00730]
  
−
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.8.4.8 1.8.4.8]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.5.1.47 2.5.1.47]  
  
 
=== Additional information===
 
=== Additional information===
Line 88: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[cysH]]-[[cysP]]-[[sat]]-[[cysC]]-[[ylnD]]-[[ylnE]]-[[ylnF]]''
+
* '''Operon:''' ''[[cysK]]''
  
 
* '''[[Sigma factor]]:''' [[SigA]]
 
* '''[[Sigma factor]]:''' [[SigA]]
  
−
* '''Regulation:'''  
+
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed], repressed in the presence of cysteine [[CymR]]
−
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]  
 
−
** repressed in the presence of cysteine ([[CymR]])
 
−
** induced by methionine starvation ([[S-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
 
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[CymR]]: transcription repression
−
** [[S-box]]: transcription termination/ antitermination, the [[S-box]] [[riboswitch]] binds S-adenosylmethionine resulting in termination [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
 
−
** [[CymR]]: transcription repression
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 125: Line 122:
 
=References=
 
=References=
  
−
<pubmed>12107147, </pubmed>
+
<pubmed>12107147 18974048, </pubmed>
 
# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
 
# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
 +
# Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in ''Bacillus subtilis''.''J. Biol. Chem.'' '''283:''' 35551-35560. [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:14, 8 June 2009

  • Description: trigger enzyme: cysteine synthetase A and control of CymR activity

Gene name cysK
Synonyms
Essential no
Product cysteine synthetase A
Function biosynthesis of cysteine, control of CymR activity
MW, pI 32 kDa, 5.492
Gene length, protein length 924 bp, 308 aa
Immediate neighbours yacD, pabB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00730

Phenotypes of a mutant

constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to Swiss-Prot)
  • Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed in the presence of cysteine CymR
  • Regulatory mechanism: CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.J. Biol. Chem. 283: 35551-35560. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
Retrieved from "https://subtiwiki.uni-goettingen.de/wiki//index.php?title=Sandbox&oldid=88813"