Difference between revisions of "Sandbox"

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* '''Description:''' 2,4-dienoyl-CoA reductase <br/><br/>
+
* '''Description:''' transcriptional repressor, regulation of fatty acid degradation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fadH ''
+
|''fadR''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykuF''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ysiA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || 2,4-dienoyl-CoA reductase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor 
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid degradation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of fatty acid degradation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 6.495  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 6.118  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 762 bp, 254 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 582 bp, 194 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuE]]'', ''[[fadG]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fadB]]'', ''[[lcfA]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13279&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14815&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ykuF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ysiA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU14060
+
* '''Locus tag:''' BSU28550
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykuFG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lcfA-ysiAB-etfBA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13290]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12330]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Trans-2,3-didehydroacyl-CoA + NADP<sup>+</sup> = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' 2,4-dienoyl-CoA reductase subfamily (according to Swiss-Prot)
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34717 O34717]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94548 P94548]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14060]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28550]
  
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.3.1.34 1.3.1.34]
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===

Revision as of 13:16, 8 June 2009

  • Description: transcriptional repressor, regulation of fatty acid degradation

Gene name fadR
Synonyms ysiA
Essential no
Product transcriptional repressor
Function regulation of fatty acid degradation
MW, pI 21 kDa, 6.118
Gene length, protein length 582 bp, 194 aa
Immediate neighbours fadB, lcfA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YsiA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU28550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

  1. Matsuoka H, Hirooka K, Fujita Y. (2007) Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation. J Biol Chem. Feb 23;282(8): 5180-94. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed