Difference between revisions of "Sandbox"

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−
* '''Description:''' Na+-coupled MotPS flagellar stator <br/><br/>
+
* '''Description:''' RNase Mini-III <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''motS''
+
|''mrnC''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytxE''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yazC''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || motility protein (flagellar motor rotation)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || RNase Mini-III
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || motility and chemotaxis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || maturation of 23S rRNA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 10.054  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.153  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 726 bp, 242 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 429 bp, 143 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acuC]]'', ''[[motP]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysS]]'', ''[[yacO]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14950&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11871&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:ytxE_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yazC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU29720
+
* '''Locus tag:''' BSU00950
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ccpA-motPS.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltX-cysES-yazC-yacOP.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10366]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12702]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' motB family (according to Swiss-Prot)
+
* '''Protein family:''' RNase III family (but lacks the dsRNA-binding domain)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 68: Line 68:
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
−
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:''' [[RplC]] binding to the precursor 23S rRNA stimulates MrnC activity [http://www.ncbi.nlm.nih.gov/sites/entrez/19154332 PubMed]
  
 
* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39064 P39064]
+
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29720]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00950]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[ccpA]] [[motP]] [[motS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16547058 PubMed]
+
* '''Operon:''' ''[[gltX]]-[[cysE]]-[[cysS]]-[[mrnC]]-[[yacO]]-[[yacP]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
−
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
−
* '''Regulation:''' constitutive
+
* '''Regulation:''' repression by cysteine ([[T-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[T-box]], overlaps a transcription terminator upstream of ''[[cysE]]'': transcription antitermination [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Ciaran Condon]], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
 +
 +
[[David Bechhofer]], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
<pubmed>16547058, </pubmed>
+
<pubmed>19154332, </pubmed>
−
# Terahara et al. (2006) An intergenic stem-loop mutation in the Bacillus subtilis ccpA-motPS operon increases motPS transcription and the MotPS contribution to motility ''J Bacteriol.'' '''188:''' 2701-2705. [http://www.ncbi.nlm.nih.gov/sites/entrez/16547058 PubMed]
+
# Redko, Y. & Condon, C. (2009) Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease.  ''Mol. Microbiol.'' '''71:''' 1145-1154. [http://www.ncbi.nlm.nih.gov/sites/entrez/19154332 PubMed]
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:25, 8 June 2009

  • Description: RNase Mini-III

Gene name mrnC
Synonyms yazC
Essential no
Product RNase Mini-III
Function maturation of 23S rRNA
MW, pI 16 kDa, 9.153
Gene length, protein length 429 bp, 143 aa
Immediate neighbours cysS, yacO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YazC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00950

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: RNase III family (but lacks the dsRNA-binding domain)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: RplC binding to the precursor 23S rRNA stimulates MrnC activity PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

David Bechhofer, Mount Sinai School, New York, USA Homepage

Your additional remarks

References

Yulia Redko, Ciarán Condon
Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease.
Mol Microbiol: 2009, 71(5);1145-54
[PubMed:19154332] [WorldCat.org] [DOI] (I p)

  1. Redko, Y. & Condon, C. (2009) Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease. Mol. Microbiol. 71: 1145-1154. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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