Difference between revisions of "Sandbox"

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−
* '''Description:''' RNase PH<br/><br/>
+
* '''Description:''' ribosomal protein L3 <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''rph''
+
|''rplC''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || exoribonuclease PH (EC 2.7.7.56)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || ribosomal protein L3 (BL3)
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || 3'-5' exoribonuclease
+
|style="background:#ABCDEF;" align="center"|'''Function''' || translation
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 4.888  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 10.31  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 735 bp, 245 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 627 bp, 209 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ysnA]]'', ''[[gerM]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpsJ]]'', ''[[rplD]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14797&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11892&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:rph_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:rplC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU28370
+
* '''Locus tag:''' BSU01160
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10357]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11218]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate (according to Swiss-Prot) 3'-5' exoribonuclease
+
* '''Catalyzed reaction/ biological activity:''' [[RplC]] binding to the precursor 23S rRNA stimulates [[MrnC]] activity [http://www.ncbi.nlm.nih.gov/sites/entrez/19154332 PubMed]
  
−
* '''Protein family:''' RNase PH family (according to Swiss-Prot)
+
* '''Protein family:''' ribosomal protein L3P family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''Interactions:''' [[YsxC]]-[[RplC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17981968 PubMed]
  
 
* '''Localization:'''
 
* '''Localization:'''
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=== Database entries ===
 
=== Database entries ===
  
−
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1OYS 1OYS]
+
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P28619 P28619]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42920 P42920]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28370]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU01160]
  
−
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.56 2.7.7.56]
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
Line 113: Line 115:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
−
 
−
[[David Bechhofer]], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
 
−
 
−
[[Ciaran Condon]], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
<pubmed>17576666 15805522, </pubmed>
+
<pubmed>19154332, </pubmed>
−
# Yao, S., Blaustein, J. B. & Bechhofer, D. H. (2007). Processing of ''Bacillus subtilis'' small cytoplasmic RNA: evidence for an additional endonuclease cleavage site. Nucleic Acids Res. 35:4464-4473. [http://www.ncbi.nlm.nih.gov/sites/entrez/17576666 PubMed]
+
# Redko, Y. & Condon, C. (2009) Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease. ''Mol. Microbiol.'' '''71:''' 1145-1154. [http://www.ncbi.nlm.nih.gov/sites/entrez/19154332 PubMed]
−
# Oussenko, I. A., Abe, T., Ujiie, H., Muto, A. & Bechhofer, D. H. (2005). Participation of 3'-to-5' exoribonucleases in the turnover of ''Bacillus subtilis'' mRNA. J Bacteriol 187:2758-2767. [http://www.ncbi.nlm.nih.gov/sites/entrez/15805522 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:34, 8 June 2009

  • Description: ribosomal protein L3

Gene name rplC
Synonyms
Essential yes PubMed
Product ribosomal protein L3 (BL3)
Function translation
MW, pI 22 kDa, 10.31
Gene length, protein length 627 bp, 209 aa
Immediate neighbours rpsJ, rplD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RplC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU01160

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RplC binding to the precursor 23S rRNA stimulates MrnC activity PubMed
  • Protein family: ribosomal protein L3P family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yulia Redko, Ciarán Condon
Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease.
Mol Microbiol: 2009, 71(5);1145-54
[PubMed:19154332] [WorldCat.org] [DOI] (I p)

  1. Redko, Y. & Condon, C. (2009) Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease. Mol. Microbiol. 71: 1145-1154. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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