Difference between revisions of "Sandbox"

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−
* '''Description:''' similar to gluconate 5-dehydrogenase <br/><br/>
+
* '''Description:''' secreted peptide, controls LiaRS activity <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''yxjF''
+
|''yydF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || secreted peptide
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of LiaRS activity
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 7.89  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 9.567  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 771 bp, 257 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 147 bp, 49 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxjG]]'', ''[[scoB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yydG]]'', ''[[fbp]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15923&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16055&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|-
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yydF_context.gif]]
−
|-
 
−
|colspan="2" | '''Genetic context''' <br/> [[Image:yxjF_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU38970
+
* '''Locus tag:''' BSU40180
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yxjC-scoAB-yxjF.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yydFGHIJ.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11155]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11479]
  
 
=== Additional information===
 
=== Additional information===
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42317 P42317]
+
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38970]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU40180]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[yxjC]]-[[scoA]]-[[scoB]]-[[yxjF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10746760 PubMed]
+
* '''Operon:''' ''[[yydF]]-[[yydG]]-[[yydH]]-[[yydI]]-[[yydJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
−
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
−
* '''Regulation:''' repressed by glucose (4-fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
+
* '''Regulation:''' activated after transition to stationary phase([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
−
* '''Additional information:'''
+
* '''Additional information:'''  
 +
**  the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
 +
** the ''yydF'' portion of the mRNA belongs to the most stable mRNAs in ''B. subtilis'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
 
=Biological materials =
 
=Biological materials =
  
−
* '''Mutant:''' GP1112 (spc), available in [[Stülke]] lab
+
* '''Mutant:'''
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
  
−
<pubmed>12850135, </pubmed>
+
<pubmed>17921301, </pubmed>
−
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
+
# Butcher BG, Lin YP, Helmann JD (2007) The ''yydFGHIJ'' operon of ''Bacillus subtilis'' encodes a peptide that induces the LiaRS two-component system''J. Bacteriol'' '''189:''' 8616-8625. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:54, 8 June 2009

  • Description: secreted peptide, controls LiaRS activity

Gene name yydF
Synonyms
Essential no
Product secreted peptide
Function control of LiaRS activity
MW, pI 5 kDa, 9.567
Gene length, protein length 147 bp, 49 aa
Immediate neighbours yydG, fbp
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YydF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU40180

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: activated after transition to stationary phase(AbrB) PubMed
  • Regulatory mechanism: AbrB: transcription repression PubMed
  • Additional information:
    • the mRNA is very stable (half-life > 15 min) PubMed
    • the yydF portion of the mRNA belongs to the most stable mRNAs in B. subtilis PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Butcher BG, Lin YP, Helmann JD (2007) The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component systemJ. Bacteriol 189: 8616-8625. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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