Difference between revisions of "Sandbox"

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−
* '''Description:''' zinc transporter<br/><br/>
+
* '''Description:''' glucose 6-phosphate dehydrogenase, pentose-phosphate pathway <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''zosA''
+
|''zwf''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykvW''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqjJ ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || P-type zinc-transporting ATPase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || glucose 6-phosphate dehydrogenase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || zinc uptake
+
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of the pentose phosphate pathway
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 68 kDa, 4.934  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.28  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1911 bp, 637 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1467 bp, 489 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[stoA]]'', ''[[ykvY]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnz]]'', ''[[gndA]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13258&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14317&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:ykvW_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:zwf_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU13850
+
* '''Locus tag:''' BSU23850
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/zwf.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13325]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11739]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' ATP + H<sub>2</sub>O + Zn<sup>2+</sup>(In) = ADP + phosphate + Zn<sup>2+</sup>(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''Catalyzed reaction/ biological activity:''' D-glucose 6-phosphate + NADP<sup>+</sup> = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)  
  
−
* '''Protein family:''' Type IB subfamily (according to Swiss-Prot)
+
* '''Protein family:''' glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31688 O31688]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P54547 P54547]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13850]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23850]
  
−
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.6.3.5 3.6.3.5]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.49 1.1.1.49]
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''zosA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''Operon:''' ''zwf'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
  
−
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''[[Sigma factor]]:'''  
  
−
* '''Regulation:''' induced by hydrogen peroxide ([[PerR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
  
−
* '''Regulatory mechanism:''' [[PerR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
−
<pubmed>12180919, </pubmed>
+
<pubmed>12850135, </pubmed>
−
# Gaballa, A.and Helmann, J. D. (2002) A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in ''Bacillus subtilis''. ''Mol. Microbiol.'' '''45:''' 997-1005. [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
# Blencke, H.-M., Homuth, G., Ludwig, H., Mäder, U., Hecker, M. & Stülke, J. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab. Engn. 5: 133-149. [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:55, 8 June 2009

  • Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

Gene name zwf
Synonyms yqjJ
Essential no
Product glucose 6-phosphate dehydrogenase
Function initiation of the pentose phosphate pathway
MW, pI 55 kDa, 5.28
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours rnz, gndA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Zwf context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: constitutive PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

  1. Blencke, H.-M., Homuth, G., Ludwig, H., Mäder, U., Hecker, M. & Stülke, J. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab. Engn. 5: 133-149. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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