Difference between revisions of "Pta"

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=The gene=
 
=The gene=

Revision as of 15:11, 22 June 2009

  • Description: phosphotransacetylase

Gene name pta
Synonyms ipa-88d, ywfJ
Essential no
Product phosphotransacetylase
Function overflow metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 34 kDa, 4.649
Gene length, protein length 969 bp, 323 aa
Immediate neighbours cysL, ywfI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Pta context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU37660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)
  • Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1XCO (complex with acetylphosphate), 1TD9
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: expression activated by glucose (2.6 fold) (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

E Presecan-Siedel, A Galinier, R Longin, J Deutscher, A Danchin, P Glaser, I Martin-Verstraete
Catabolite regulation of the pta gene as part of carbon flow pathways in Bacillus subtilis.
J Bacteriol: 1999, 181(22);6889-97
[PubMed:10559153] [WorldCat.org] [DOI] (P p)

B S Shin, S K Choi, S H Park
Regulation of the Bacillus subtilis phosphotransacetylase gene.
J Biochem: 1999, 126(2);333-9
[PubMed:10423526] [WorldCat.org] [DOI] (P p)