PhrK

• Description: response regulator aspartate phosphatase (RapK) regulator, controls ComA activity
  
  

Gene name	phrK
Synonyms
Essential	no
Product	phosphatase (RapK) regulator
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrK
Interactions involving this protein in SubtInteract: PhrK
MW, pI	3 kDa, 8.158
Gene length, protein length	120 bp, 40 aa
Immediate neighbours	rapK, yobH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

AbrB regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU18920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibits RapK activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapK-PhrK PubMed

• Localization:

Database entries

• Structure:

• UniProt: O31840

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapK-phrK PubMed

• Expression browser: phrK PubMed

• Sigma factor: SigH PubMed

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)