Difference between revisions of "NrgA"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP168 (in [[pAC7]]), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 18:35, 29 October 2009

  • Description: ammonium transporter, required at low ammonium concentration

Gene name nrgA
Synonyms amtB
Essential no
Product ammonium transporter
Function ammonium uptake
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 42 kDa, 5.727
Gene length, protein length 1212 bp, 404 aa
Immediate neighbours ywoB, nrgB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NrgA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU36510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by ammonium limitation
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism: transcription activation by TnrA
  • Additional information:

Biological materials

  • Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab
  • Expression vector:
  • lacZ fusion: pGP168 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076] [WorldCat.org] [DOI] (P p)

Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685] [WorldCat.org] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935] [WorldCat.org] [DOI] (P p)