Difference between revisions of "AlsS"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[carbon core metabolism]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[AlsR regulon]]}},
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{{SubtiWiki regulon|[[Rex regulon]]}},
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{{SubtiWiki regulon|[[stringent response]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[carbon core metabolism]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 00:04, 9 December 2010

  • Description: acetolactate synthase

Gene name alsS
Synonyms
Essential no
Product acetolactate synthase
Function overflow metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 61 kDa, 5.164
Gene length, protein length 1713 bp, 571 aa
Immediate neighbours alsD, alsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AlsS context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

AlsR regulon, Rex regulon, stringent response

The gene

Basic information

  • Locus tag: BSU36010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: TPP enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by acetate (AlsR) PubMed
    • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
    • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

  • Regulatory mechanism:
    • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
    • AlsR: transcription activation in the presence of acetate PubMed
    • Rex: transcription repression if the ratio NADH2/NAD is high PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References