Difference between revisions of "RecA"

From SubtiWiki
Jump to: navigation, search
Line 12: Line 12:
 
|style="background:#ABCDEF;" align="center"| '''Product''' || multifunctional protein involved in homologous <br/>recombination and DNA repair ([[LexA]]-autocleavage)
 
|style="background:#ABCDEF;" align="center"| '''Product''' || multifunctional protein involved in homologous <br/>recombination and DNA repair ([[LexA]]-autocleavage)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic [[recombination]], [[DNA repair]]
+
|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.883   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.883   

Revision as of 12:15, 1 February 2011

  • Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)

Gene name recA
Synonyms recE
Essential no
Product multifunctional protein involved in homologous
recombination and DNA repair (LexA-autocleavage)
Function DNA repair/ recombination
MW, pI 37 kDa, 4.883
Gene length, protein length 1041 bp, 347 aa
Immediate neighbours cinA, pbpX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RecA context.gif
This image was kindly provided by SubtiList




Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon, LexA regulon

The gene

Basic information

  • Locus tag: BSU16940

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

RecA filaments are dismantled from DNA by PcrA PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: recA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-2 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • Nucleoid (Mid-cell) PubMed
    • localizes to one cell pole PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by DNA damage (LexA) PubMed
    • induced by conditions that trigger development of genetic competence (ComK) PubMed
  • Additional information:

Biological materials

  • Mutant: IRN444 (cat), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References

Reviews

Justin Courcelle, Philip C Hanawalt
RecA-dependent recovery of arrested DNA replication forks.
Annu Rev Genet: 2003, 37;611-46
[PubMed:14616075] [WorldCat.org] [DOI] (P p)

Michael M Cox
The bacterial RecA protein as a motor protein.
Annu Rev Microbiol: 2003, 57;551-77
[PubMed:14527291] [WorldCat.org] [DOI] (P p)

Shelley L Lusetti, Michael M Cox
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
Annu Rev Biochem: 2002, 71;71-100
[PubMed:12045091] [WorldCat.org] [DOI] (P p)

M M Cox
Recombinational DNA repair in bacteria and the RecA protein.
Prog Nucleic Acid Res Mol Biol: 1999, 63;311-66
[PubMed:10506835] [WorldCat.org] [DOI] (P p)

Original publications